Analysis Note

ControlWestern Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.

Application

Detect CNPase using this Anti-CNPase Antibody, clone 11-5B validated for use in IC, IH, IH(P) & WB.

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.

Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).

Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

Immunogen

Purified human brain CNPase

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide

Quality

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 µg of Mouse Brain lysates.

Specificity

CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

48 & 46 kDa